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Asian J Androl 5:121–124Īndersson AM, Jorgensen N, Larsen LF, Rajpert-De Meyts E, Skakkebaek NE (2004) Impaired Leydig cell function in infertile men: a study of 357 idiopathic infertile men and 318 proven fertile controls. J Complement Integr Med 7:1–10Īrti S, Pramod KV, Dixit VP (2003) Effect of Semecarpus anacardium fruits on reproductive function of male albino rats. Andrologia 18:125–131Īllouh MZ, Khouri NA, Daradka HM, Kaddumi EG (2010) Orchis anatolica root ingestion improves sexual motivation and performance in male rats. Anat Rec 94:238–248Īgrawal S, Chauhan S, Mathur R (1986) Antifertility effects of embelin in male rats. Concomitantly, an elevation in female impregnation is induced when allowed mating with treated male rats.Ībercrombie M (1946) Estimation of nuclear population from microtome section. anatolica induces positive androgenic mediated significant effects on male rat reproductive system morphology, functions, and blood biochemical markers. When controlled female rats were allowed mating with treated male rats (two females to one male), the number of intra-uterine implantations and viable fetuses were enhanced in impregnated females. Blood biochemical markers such as blood serum glucose and cholesterol were reduced whereas significant elevation were detected in both testosterone and follicle-stimulating hormone titers ( P < 0.01) in all treated rat groups.
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Elevation in the percentage of sperm motility ( P < 0.01), density (million/ml) ( P < 0.01) with an increase in Leydig cell nuclear diameter, and seminiferous tubes diameter in both treated groups were noted. Interstitial cell line count including fibroblast, immature, and mature Leydig cells were also elevated in response to elevated treatment dose ( P < 0.01). Germinal cell line count response to treatment was noted and manifested by significant elevation in spermatogonia, primary and secondary spermatocytes ( P < 0.05 and P < 0.01), and spermatid ( P < 0.01) especially when the high dose was used. Results indicate a dose-dependent increase in testicular, seminal vesicle, and epididymis weights (low dose P < 0.05 and high dose P < 0.01). anatolica root bulbs powder orally for 60 days that were compared with a control group. One group received low daily doses of 500 mg and the other received high dose of 1,000 mg/kg of O. Two treatment groups of rats (ten each) were used in this experiment. Treatment effects of dried Orchis anatolica root bulbs on male Sprague–Dawley rats' reproductive system, fertility, and biochemical markers were investigated.
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